dynamic light scattering data interpretation

Hydrodynamic sizes are more easily measured than radii of gyration and can be measured over a wider range of sizes. The conversion from hydrodynamic radius to radius of gyration is a function of chain architecture (including questions of random coil vs. The hydrodynamic radius is not the same as the radius of gyration. Radius calculations are the same except for a factor of two.Īlso, a note to those interested in polymer size. When experimental data are uploaded to the server they are processed in a few seconds, and the result is displayed on the screen in the form of a size. That is, the determined particle size is the size of a sphere that diffuses the way as your particle.įor those who work with protein sizing and other areas where hydrodynamic radius is more commonly used, note that the development here is around diameter. Finally, and most importantly, it reminds the analyst that the particle size determined by dynamic light scattering is the hydrodynamic size. The parameters are important characteristics mainly for drug delivery system (DDS) formulations, such as solid in oil (S/O) formulations, liposome formulations, suspension formulations. Temperature is even more important due to the viscosity term since viscosity is a stiff function of temperature. Dynamic light scattering (DLS) is used for measuring average particle diameter and particle diameter distribution of nano-sized particles dispersed in liquid. The first is that sample temperature is important, at it appears directly in the equation.

However, the equation does serve as important reminder about a few points. The calculations are handled by instrument software. T is thermodynamic temperature (we control this) This paper reviews dynamic light scattering (DLS) of gold nanoparticle-protein interactions for the model protein bovine serum albumin (BSA), as well as in complex biofluids, at the example of mouse serum.k B is Boltzmann’s constant (we know this).D t is the translational diffusion coefficient (we find this by dynamic light scattering).The following discussion reviews some of the basic concepts of dynamic light scattering and outlines some of the pitfalls that are often encountered in data interpretation. D h is the hydrodynamic diameter (this is the goal: particle size!) As well, dynamic light scattering results are often open to misinterpretation if one is unaware of the state of the sample and the method of data analysis.

Dynamic light scattering data interpretation